Mehadi Hasan Rony1*, Md. Abu Sayeed Imran1, Rabiul Islam1, Faisal Ahmed2, Bushra Binte Zaker3, Pinki Akter4, Md. Golam Mosaib5, Rintu Kumar Sarker1, and Md. Ramjan Sheikh5
1Dept. of Biotechnology and Genetic Engineering, Islamic University, Bangladesh; 2Dept. of Pharmacy, University of Asia Pacific, Dhaka, Bangladesh; 3Dept. of Mathematics and Natural Sciences, Biotechnology Program, BRAC University, Dhaka, Bangladesh; 4Dept. of Microbiology, Stamford University Bangladesh, Dhaka, Bangladesh; and 5Dept. of Biochemistry and Molecular Biology, Gono Bishwabidyalay, Dhaka, Bangladesh
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Pathogenic microorganisms are major health concerns of infectious diseases. In the present study ethanolic and methanolic extracts of Cassia obtusifolia leaves from the Kushtia region (Bangladesh) were subjected to evaluate the in vitro microbial activity against six important human pathogenic bacteria viz., Bacillus subtilis (001-1), Sarcina lutea (002-1), Xanthomonas campestris (004-1), Escherichia coli (005-1), Klebsiella pneumonia (006-1) and Pseudomonas sp. (010-1) employing disc diffusion method. The crude methanolic extract of C. obtusifolia produced a maximum area of inhibition (14 mm) against S. lutea (002-1) and the crude ethanolic extract of C. obtusifolia produced the largest area of inhibition (11 mm) against K. pneumonia (006-1). The MIC values (256 μg/ml, 512 μg/ml) were obtained from the methanolic isolate and ethanolic extract that produced 4 mm and 3 mm area of inhibition against S. lutea (002-1) and K. pneumonia (006-1). The methanol extract showed greater activity than the ethanol extract. The most susceptible bacterial strains to ethanol and methanol extracts were S. lutea (002-1) and K. pneumonia (006-1). So, it may be possible that the production of a new antibiotic from C. obtusifolia L. leaf may be recommended for meningitis and pneumonia. The findings of this research suggest that the extracts of C. obtusifolia L. can be a source of natural antibacterial agents with pivotal applications in pharmaceutical companies to control pathogenic bacteria causing severe illness in humans.
Keywords: Cassia obtusifolia, Antibacterial effect, Pathogenic bacteria, Leaf extract, and Disease management.
Citation: Rony MH, Imran MAS, Islam R, Ahmed F, Sarker RK, Zaker BB, Akter P, Mosaib MG, and Sheikh MR (2019). Determination of antimicrobial activity of medicinal plant Cassia obtusifolia L. (Chakunda) leaf extract on selected pathogenic microbes, Am. J. Pure Appl. Sci., 1(6), 59-69. https://doi.org/10.34104/ajpab.019.01959069
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