Mohammad Zakerin Abedin1*, Md. Sadiqur Rahman2, Rubait Hasan3, Jamiatul Husna Shathi4, Laila Jarin5, and Md. Sifat Uz Zaman6
1Dept. of Microbiology, Khwaja Yunus Ali University, Sirajgonj, Bangladesh; 2Dept. of Microbiology, Aqua Laboratory Quality Feeds Limited, Mymensingh, Bangladesh; 3&4Dept. of Biochemistry and Biotechnology, Khwaja Yunus Ali University, Sirajgonj, Bangladesh; 5Dept. of Microbiology, LabAid Medical Centre Gulshan Ltd, Dhaka, Bangladesh; and 6Dept. of Microbiology, Popular Diagnostic Centre Ltd, Dhaka, Bangladesh.
*Correspondence: zakerin.du2016@gmail.com (Mohammad Zakerin Abedin, Assistant Professor, Dept. of Microbiology, Khwaja Yunus Ali University, Bangladesh)
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ABSTRACT
Bacterial diseases are widespread and can be of particular importance in the fish farming of Bangladesh. This investigation was done to assess and compare the bacteria diversities and population in local fresh water pond fishes. Out of 95 samples, 54(56.9%) were Shing (Heteropneustes fossils), 14(14.7%) were Pangas (Pangasius pangasius), 9(9.5%) were Pabda (Ompok spp), 7(7.3%) were Thai Koi, (Anabas testudineus), and 11(11.6%) were others infected fishes such as Shol (Channa striata), Magur (Clarias spp), Tilapia (Oreochromis niloticus), and Tengara (Mystus cavasius) fishes. Among 95 infected fishes 84(88.4%) were infected with pathogenic bacteria and 11(11.6%) were normal flora. There were eight types of different isolated spp with the frequency of occurrence were 36 (42.9%) Aeromonas spp, 15 (17.9%) Pseudomonas spp, 7(8.3%) Vibrio spp, 9 (10.7%) Staphylococcus spp, 7(8.3%) Flavobacterium spp, 7(8.3%) Edwardsiella spp, others were 3(3.6%) Citobacter spp, and Enterobacter spp respectively. All of the isolated pathogenic bacteria showed 84/84(100%) resistant to Amoxicillin and 18/84(64.3%) resistance to Erythromycin. All the strains showed sensitivity to Ciprofloxacin, Cotrimoxazole, Enorfloxacin, Doxyciline, Clotetracycline, and Colistin with the frequency of occurrence were 78/84(92.8%), 76/84 (90.5%), 73/84(86.9%), 67/84(67.9%), 53/84(63.1%) and 52/84(61.9%) respectively. The physiochemical characteristics of 95 pond water samples were analyzed; the average water temperature, pH, and ammonia were 27.3oC, 7.6, and 0.87mg/L respectively. A significant variation in the physiochemical parameters like water temperature, pH, ammonia was observed within these five types of pond water. Hence, it is important to detect fish diseases, responsible pathogens, and other agents for the protection of our water resources.
Keywords: Fresh water fishes, Pathogenic microorganisms, antibiotic sensitivity, physiochemical parameters.
Citation: Abedin MZ, Rahman SM, Hasan R, Shathi JH, Jarin L, and Sifat Uz Zaman M. (2020). Isolation, identification, and antimicrobial profiling of bacteria from aquaculture fishes in pond water of Bangladesh. Am. J. Pure Appl. Sci., 2(3), 39-50. https://doi.org/10.34104/ajpab.020.039050
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