img
img
img
img
img
img
Home / all-journals/ /Article

Effects of Phytohormone and Regulators on Shoot Tip and Nodal Explants for In Vitro Shoot and Root Clonal Propagation of Vitex negundo L.


Safia Iqbal1,3, A.K.M. Nazmul Huda1, A.N.K. Mamun2, Md. Rezuanul Islam1, Mohammad Abu Hena Mostofa Jamal1, and Md. Rezaul Karim1*

1Dept. of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh; 2Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, Atomic Energy Commission, Gonakbari, Savar, Dhaka, Bangladesh and 3SunUp International School and College, Kushtia 7000, Bangladesh. 

*Correspondence: rezaulkarim@btge.iu.ac.bd (Md. Rezaul Karim, Assistant Professor, Dept. of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh).

Powered by Froala Editor


ABSTRACT

Medicinal plants are one of the most vital natural resources, but many of them are currently endangered due to habitat loss. Consequently, it is critical to emphasize the importance of using micropropagation techniques for mass propagation of plantlets on a commercial scale, in addition to germplasm conservation and distribution. Nodal explants and shoot tips were expunged from 15 days of the explant by aseptic seedlings, an effective, quick, and better in vitro plant regeneration procedure for Vitex negundo L. has been developed. The recent study was considered to develop an in vitro procedure for the regeneration of V. negundo L., a traditional medicinal plant. Nodal segments and shoot tips were cultivated on MS medium enhanced with numerous plant growth regulators. For multiple shoots and root regeneration, various cytokinins were examined. 6-benzyl-aminopurin (BAP), kinetin (Kin), and 1H-indole-3-butanoic acid (IBA) were all tested as a supplement to Murashige and Skoog (MS) medium including auxin phytohormone, such as Indole acetic acid (IAA) and 1-naphthaleneacetic acid (NAA). The furthermost effective surface sterilization treatment for explants of V. negundo has been found 0.1% HgCl2 for 8 minutes. In all treatments, multiple shoots were collected from shoot tips and nodal segments. In MS media added with 2.0mg/l BAP, the most shoots were seen in V. negundo. Furthermore, V. negundo regeneration shoots rooted effectively in half MS containing 1.0 mg/l IBA. Finally, proliferated plantlets were effectively adapted in soil, where they grew normally without morphological anomalies and had a survival rate of 92 percent.

Keywords: In vitro regeneration, Medicinal plant, Vitex negundo, Phytohormone, and Growth regulators.

Citation: Iqbal S, Huda AKMN, Mamun ANM, Islam MR, Jamal MAHM, and Karim MR. (2021). Effects of phytohormone and regulators on shoot tip and nodal explants for in vitro shoot and root clonal propagation of Vitex negundo L. Am. J. Pure Appl. Sci., 3(4), 65-78. https://doi.org/10.34104/ajpab.021.065078


Powered by Froala Editor