Abu Saim Mohammad Saikat1*and Abul Bashar Ripon Khalipha2,3
1Dept. of Biochemistry and Molecular Biology, Bangabandhu Sheikh Mujibur Rahman Science and Technology University (BSMRSTU), Gopalganj, Bangladesh; 2Dept. of Pharmacy, Bangabandhu Sheikh Mujibur Rahman Science and Technology University (BSMRSTU), Gopalganj, Bangladesh; 3Evergreen Scientific Research Centre, Dept. of Pharmacy, Bangabandhu Sheikh Mujibur Rahman Science and Technology University (BSMRSTU), Gopalganj, Bangladesh.
*Correspondence: asmsaikat.bmb@gmail.com (Abu Saim Mohammad Saikat, Dept. of Biochemistry and Molecular Biology, BSMRSTU, Gopalganj, Bangladesh).
Powered by Froala Editor
ABSTRACT
Bacillus cereus is enteropathogenic and widely distributed pathogen in the environment, which is mainly associated with food poisoning. In the intestine, B. cereus produces enterotoxins resulting in diarrhoea, abdominal distress and vomiting, and a range of infections in humans. BCRIVMBC126_02492 is a functional protein of B. cereus, which is related to oxidation glutathione persulfide in the mitochondria, cyanide fixation, and also has a variety of biological functions. Nevertheless, protein BCRIVMBC126_02492 is not explored. Therefore, the structure prediction, functional annotation, and characterization of the protein are proposed in this study. Modeller, Swiss-model, and Phyre2 are used for generating tertiary structures. The structural quality assessment of the protein determined by Ramachandran Plot analysis, Swiss-Model Interactive Workplace, and Verify 3D tools. Furthermore, Z-scores applied to detect the overall tertiary model quality of the protein. A comparison of the results showed that the models generated by Modeller were more suitable than Phyre2 and Swiss Models. This investigation decoded the role of this unexplored protein of B. cereus. Therefore, it can bolster the way for enriching our knowledge for pathogenesis and drug and vaccine targeting opportunities against B. cereus infection.
Keywords: Bacillus cereus, Protein BCRIVMBC126_02492, Functional annotation, and Pathogenesis.
Citation: Saikat ASM, and Khalipha ABR. (2020). Structure prediction, characterization, and functional annotation of uncharacterized protein BCRIVMBC126_02492 of Bacillus cereus: an in silico approach. Am. J. Pure Appl. Sci., 2(4), 104-111. https://doi.org/10.34104/ajpab.020.01040111
Powered by Froala Editor