Usman Ahmad1*, Abdul Mateen1, Naveed Iqbal Raja2 and Zia-ur-Rehman Mashwani2
1Department of Biology, PMAS Arid Agriculture University Rawalpindi - 46300, Pakistan; and 2Department of Botany, PMAS Arid Agriculture University Rawalpindi - 46300, Pakistan.
*Correspondence: usmanahmad239@yahoo.com (Usman Ahmad, SST-Biology, Federal Directorate of Education, Islamabad, Pakistan).
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ABSTRACT
In the present study, the evaluation of mineral contents of black pepper seeds is determined by atomic absorption spectrometry. The mineral analysis of the Atomic Absorption Spectrometry (AAS) of black pepper consists of Manganese (Mn) 2.282 ± 0.01 µg/ml, Magnesium (Mg) 8.148 ± 0.08 µg/ml, Copper (Cu) 0.27 ± 0.005 µg/ml, Zinc (Zn) 0.593 ± 0.01 µg/ml and Iron (Fe) 0.89 ± 0.005 µg/ml. The efficiency and stability of natural antioxidants in black pepper seed oil are determined by reducing power (FRAP). Free radicals present in oils and fats are responsible for harmful for the body cell. The stability of natural antioxidants of black pepper seed oil and their significance at different concentrations is calculated by the One Way ANOVA Test. In the present study, the result showed that the increased concentration of black pepper seed oil by solvent soxhlet extraction and cold pressing (25 µg/ml - 100 µg/ml) has a positive correlation with reducing power assay. The result showed that black pepper oil can remove toxic compounds produced in the body and help maintain the body cells. In addition, the extraction methods can be helpful for the improvement in the preservation of important nutrients that may be sensitive to thermal heat. Cold pressing is a useful method as it can exclude the use of heat as well as organic solvent in the soxhlet method.
Keywords: Mineral contents (AAS), Reducing assay (FRAP), and One-way ANOVA Test.
Citation: Ahmad U, Mateen A, Raja NI, and Mashwani ZUR. (2024). Evaluation of mineral contents and antioxidant ability of black pepper seed oil by soxhlet and cold pressing. Am. J. Pure Appl. Sci., 6(4), 113-120. https://doi.org/10.34104/ajpab.024.01130120
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